Determination of Chromatographic Elution Profiles Using Non-normalized Singular Value Evolving Profiles

✍ Scribed by Nickey J. Messick; John H. Kalivas

• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 152 KB
• Volume: 55
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1996.1414

✦ Synopsis

An approach to determining component-wise chromatographic elution profiles is proposed. The method uses non-normalized spectra from a spectrochromatogram and subsequent singular value decompositions of these data to create second singular value evolving profiles (SVEP2s). The SVEP2s are shown to approximate actual elution profiles for all components in a sample. Gas chromatography Fourier transform infrared spectroscopy, liquid chromatography with photodiode array detection, and simulated spectrochromatograms are used to test the effectiveness of this method. The high correlations found between estimated elution profiles and actual chromatograms affirms the ability of the approach.