Determination of choline in erythrocytes using high-resolution proton nuclear magnetic resonance spectroscopy: Comparison with a choline oxidase method
✍ Scribed by Georg R. Beilharz; Carl R. Middlehurst; Philip W. Kuchel; Glenn E. Hunt; Gordon F.S. Johnson
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 426 KB
- Volume
- 137
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Detailed operating conditions are reported for the determination of choline in human erythrocytes using proton nuclear magnetic resonance spectroscopy in conjunction with the inversion-recovery spin-echo pulse sequence. The results of the NMR method were in excellent agreement with those obtained using an enzymatic (choline oxidase) assay; however, they were approximately three times higher than those reported using gas chromatography/mass spectrometry techniques. The differences may be partly due to the method of preparing or sampling cells since there is a distribution of choline in cells of different ages. However, choline levels were not affected by the methods used in the present study for storing or preparing cells.