Determination of chloramphenicol at ultra-trace levels by high-performance differential pulse polarography: Application to Milk and Meat

✍ Scribed by J.J. VanDer Lee; W.P. Van Bennekom; H.J. De Jong

• Publisher: Elsevier Science
• Year: 1980
• Tongue: English
• Weight: 979 KB
• Volume: 117
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(80)87016-4

✦ Synopsis

High

-performance differential pulse polarography (h.p.d.p.p.) is used to determine the antibiotic chloramphenicol. Optimum operating conditions are discussed. Calibration curves are linear from 10 ng ml-' to 1 pg ml-'; the detection limit is about 3 ng ml". Above 1 pg ml.', deviations from linearity occur, because of adsorption of chloramphenicol at the mercury electrode. A correction, baaed on variations III natural drop time, issuggested. Chloramphenicol is readily extracted from milk or minced meat with diethyi e&her_ The ether is evaporated, and the residue is taken up in acetate buffer pH 4.7 and filtered through Keagtrttts and apparatus ChloramphenicoI (Globenicoi, Gist-Brocades, Defft) was used as received: h.p.1.c. did not reveal degradation products (on Lichrosorb RP S, 5 Hrn, with