Determination of Buprenorphine in Pharmaceuticals and Human Urine by Adsorptive Stripping Voltammetry in Batch and Flow Systems

✍ Scribed by M. Angeles García-Fernández; M. Teresa Fernández-Abedul; Agustín Costa-García

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 157 KB
• Volume: 12
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/(sici)1521-4109(200005)12:7<483::aid-elan483>3.0.co;2-q

✦ Synopsis

The adsorption of buprenorphine on carbon paste electrodes has been studied by cyclic (CV) and differential pulse voltammetry (DPV). By applying a simple electrode pretreatment good reproducibility of the current signal is obtained (RSD 0.85 %, n 6 in CV and RSD 0. 35 %, n 6 in DPV for a 5.0610 À7 M buprenorphine concentration in BR buffer pH 9.0). These methods permit a very sensitive (LD 2.0610 À8 M) and selective determination. They were applied to the quantitation of buprenorphine in human urine coming from subjects following buprenorphine treatment. The methodology was coupled to a ¯ow system incorporating a thin-layer ¯ow cell. A linear response was obtained in the concentration range comprised between 1.0610 À8 and 8.0610 À7 M. The application to the determination of this molecule in pharmaceuticals (vials and tablets) and human urine without any sample pretreatment is studied.