Determination of brazilein in rat plasma after intravenous administration by HPLC

✍ Scribed by Jiaqi Lan; Jun Hu; Bonan Li; Dongming Xing; Chengcheng Liu; Wei Wang; Lijun Du

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 153 KB
• Volume: 22
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1045

✦ Synopsis

Abstract

Quantification of brazilein in rat plasma following intravenous administration was achieved by reversed‐phase high‐performance liquid chromatography using a mobile phase of acetonitrile–0.05 m potassium dihydrogen phosphate water (containing 0.5% triethylamine, pH 3.0; 20:80 v/v) and UV detection at 445 nm. The method was linear (determination coefficient, r^2^ = 0.9992) within the tested range (0.313–5.0 µg/mL). Intra‐ and inter‐day precision coefficients of variation and accuracy bias were acceptable (maximal CV value was 2.06% for intra‐day and 1.71% for inter‐day) over the entire range. The recoveries were 81.48, 84.61 and 82.83% for concentrations of 0.313, 1.25 and 5.0 µg/mL, respectively. The concentration–time curve of brazilein after intravenous administration was fitted to the two‐compartment model. This is the first time that brazilein in rat plasma was detected by HPLC‐UV method and its pharmacokinetic characteristic was comprehensively studied. Copyright © 2008 John Wiley & Sons, Ltd.