Determination of biotin in low-temperature fish-meal processed from different species by means of a microbiological method usingLactobacillus plantarum as test organism
✍ Scribed by M�land, Anne; Sandnes, Kjartan
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 69 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0022-5142
No coin nor oath required. For personal study only.
✦ Synopsis
Determination of biotin content in 10 ®sh-meals produced from different species was performed by a microbiological assay using Lactobacillus plantarum as test organism. The contents of biotin in the ®sh-meal samples ranged between 0.36 and 0.80 mg kg À1 . Most of the ®sh-meals contained approximately 0.4 mg biotin kg À1 . The highest value was found in meal processed from sand eel (Ammodytes marinus). The precision of the assay showed a 2.7% relative standard deviation. Recovery using addition of biotin to the ®sh-meal ranged between 93 and 95%. Testing of extraction procedure suggested that hydrolysis in 1.5 M H 2 SO 4 at 121 °C for 3 h is suf®cient for biotin to be liberated from proteins and amino acids.