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Determination of binding parameters of cyclic AMP and its analogs to cyclic AMP-dependent protein kinase by the fluorescent probe method

โœ Scribed by A.G. Gabibov; S.N. Kochetkov; L.P. Sashchenko; E.S. Severin


Book ID
118308993
Publisher
Elsevier Science
Year
1979
Weight
721 KB
Volume
569
Category
Article
ISSN
0005-2744

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Effects of glucagon and insulin on the c
โœ Carlos J. Ciudad; Jordi Vila; M.Angels Mor; Joan J. Guinovart ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Springer ๐ŸŒ English โš– 567 KB

Extracts obtained from rat hepatocytes incubated with saline, glucagon or insulin were electrophoresed on polyacrylamide gels and then assayed for cyclic (3H)AMP binding capacity. Analysis of the binding patterns demonstrated that glucagon dissociated a holoenzyme of cyclic AMP-dependent protein kin