Determination of benzodiazepines in clinical serum samples: Comparative evaluation of REMEDi system, aca analyzer, and conventional HPLC performance
✍ Scribed by Tsui-Ling Chang; Kuan-Wen Chen; Yih-Dar Lee; Kang Fan
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 43 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0887-8013
No coin nor oath required. For personal study only.
✦ Synopsis
Emergency toxicology or drug screening in clinical settings requires rapid qualitative and quantitative analysis with acceptable levels of sensitivity and specificity. The aim of this study was to comparatively evaluate the performance of the multi-column HPLCbased REMEDi drug profiling system (Bio-Rad), the aca analyzer (Du Pont), and the bench standard conventional HPLC method in the identification of 12 clinically important benzodiazepines. In this study, the presence of benzodiazepines in 133 patients' serum samples were qualitatively and comparatively analyzed by these three procedures. Among these methods, 120 of 133 samples were identified as benzodiazepine-positive by conventional HPLC (90%); 127 by aca analyzer (95%); and 84 by REMEDi (63%). Detection sensitivity of REMEDi for most of the benzodiazepines was found satisfactory when concentrations were greater than 1.0 µg/mL. When benzodiazepine concentrations were in the ranges of 0.3-1.0 µg/mL, detection sensitivity became varied among the benzodiazepine family of drugs by REMEDi method. REMEDi procedure should not be considered as the method of choice 1999.