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Determination of Ascorbic Acid and Dehydroascorbic Acid in Biological Samples by High-Performance Liquid Chromatography Using Subtraction Methods: Reliable Reduction with Tris[2-carboxyethyl]phosphine Hydrochloride: Volume 282, Number 1 (2000), pages 89–93

✍ Scribed by Jens Lykkesfeldt


Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
16 KB
Volume
291
Category
Article
ISSN
0003-2697

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✦ Synopsis


On page 91, column 1, lines 10 -18, the mobile phase description contained errors as printed. The correct mobile phase composition is printed here: One liter of mobile phase consisted of 17.8 g sodium dihydrogen phosphate monohydrate (129 mM), 17.6 g sodium acetate anhydrous (215 mM), 372 mg disodium-EDTA (1.0 mM), 50 mg n-dodecyltrimethylammonium chloride (189 M) in 750 ml H 2 O mixed with 20 mg tetraoctylammonium bromide (36.6 M) dissolved in 250 ml methanol and the pH of the mixture was adjusted to 5.4 with ortho-phosphoric acid. The author sincerely regrets any confusion that this error may have caused.

This erratum is


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Determination of Ascorbic Acid and Dehyd
✍ Jens Lykkesfeldt 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 68 KB

Determination of dehydroascorbic acid in biological samples most commonly involves indirect measurement. The concentration is calculated by subtraction of the measured ascorbic acid concentration from that of total ascorbic acid analyzed after reduction of the dehydroascorbic acid present; a methodo