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Determination of apparent kinetic parameters for competitive product inhibition in packed-bed immobilized enzyme reactors

✍ Scribed by Ahmet R. Özdural; Deniz Tanyolaç; İsmail H. Boyacı; Mehmet Mutlu; Colin Webb

Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
215 KB
Volume
14
Category
Article
ISSN
1369-703X

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✦ Synopsis

In this study, a simple and effective technique for characterizing Michaelis-Menten type kinetics with competitive product inhibition in packed-bed re-circulated immobilized enzyme reactors is presented, where the use of nonlinear regression techniques for multi-parameter estimation are not required. In order to demonstrate the new technique introduced in this work, enzymatic conversion of lactose in a recycling packed-bed reactor is envisaged where ␤-galactosidase (lactase, EC 3.2.1.23) enzyme is immobilized on a weak base ion exchanger resin (Duolite A 568). For the experimental conditions used in this research, the total competitive inhibition by product (galactose) model is sufficient to represent the lactose hydrolysis kinetics in a packed-bed reactor.

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