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Determination of anti-Aspergillusactivity of antifungal agents based on the dynamic growth rate of a single hypha

✍ Scribed by Kibong Oh; Hideaki Matsuoka; Yasuyuki Nemoto; Osao Sumita; Kosuke Takatori; Hiroshi Kurata

Book ID
104657885
Publisher
Springer
Year
1993
Tongue
English
Weight
461 KB
Volume
39
Category
Article
ISSN
1432-0614

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✦ Synopsis

The dynamic growth rate of a single hypha of Aspergillus niger was analysed using an automatic system. A colony of A. niger was in contact with saline, saline containing an antifungal agent, and flushing saline, in sequence. The growth rate of a test hypha selected arbitrarily from the colony responded dynamically to the antifungal agent. The minimum concentration that caused the complete inhibition of hyphal growth was defined as the minimum inhibitory concentration (MIC). The MIC values obtained were compared with those determined by conventional methods based on increasing rate of colony diameter or dry matter weight.

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