Determination of amprolium in feed stuff by capiliary isotachophoresis
✍ Scribed by Ludmila Křivánková; Dr. Petr Boček
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 206 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0173-0835
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✦ Synopsis
A method for the determination of amprolium in feed stuff by analytical capillary isotachophoresis with column coupling is described. Up to 20 PI of the raw water extract of amprolium from a feed stuff can be analysed directly without any pretreatment in 15 min. The detection limit reached the level of ppm, the reproducibility of analyses in the followed range of 0.2-1.2 nmoles was 3 re]. %. Amprolium, 1-(4-amino-2-propyl-5-pyrimidinylmethyl)-2methylpyridinium chloride hydrochloride (see Fig. l), is an efficient veterinary drug for the treatment and prevention ofoutbreaks of coccidiosis in poultry. Its activity consists in blocking the uptake of vitamine B , by coccidia, which require much more vitamine B , than poultry 11 I. When amprolium is not ad-
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