Determination of aliphatic carboxylic acids in non-aqueous matrices by liquid chromatography with peroxyoxalate chemiluminescence detection

✍ Scribed by Anthony Gachanja; Paul Worsfold

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 598 KB
• Volume: 290
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)80059-6

✦ Synopsis

A peroxyoxalate chemiluminescence (CL) method for the qualitative and quantitative analysis of used engine oils for aliphatic carboxylic acids (C,-C,) is presented. The carboxylic acids were coupled to a fluorescent label, 9-anthracenemethanol, and separated using reversed-phase liquid chromatography (LC). CL reagents (bis(2,4-dinitrophenyl)oxalate and hydrogen peroxide) were mixed with the LC eluent and the CL emission detected using a solid state photodiode-based detector. Detection limits (3 X blank signal) for aliphatic carboxylic acids fC,-C,) were in the range 1.8-4.5 pmol on-column (5 ~1 injection). A comparison between fluorescence and photodiode and photomultiplier-based CL detection of 9-anthracenemethyl esters of C,-C, aliphatic carboxylic acids in a flow injection manifold is also presented.