Determination of albuterol concentrations in human plasma using solid-phase extraction and high-performance liquid chromatography with fluorescence detection

✍ Scribed by R.E. Bland; R.J.N. Tanner; W.H. Chern; J.R. Lang; J.R. Powell

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 443 KB
• Volume: 8
• Category: Article
• ISSN: 0731-7085
• DOI: 10.1016/0731-7085(90)80085-4

✦ Synopsis

A fast, simple, and accurate method for determining albuterol concentrations in human plasma has been developed and validated for use in routine clinical analyses. This method involves a solid-phase extraction procedure using silica cartridges and normal-phase high-performance liquid chromatography with fluorescence detection. Regression analysis showed the method was linear over the standard curve range 1-16 ng ml-1. The percent recovery for albuterol and the internal standard, bamethane, at 5 ng ml-1 was found to be greater than 90%. The newly developed method has been applied in the analysis of plasma samples from patients and healthy volunteers.