Determination of adenosine triphosphate in human semen to estimate the fertilizing potential and to quantify sperm antibodies
✍ Scribed by Comhaire, Frank H. ;Vermeulen, Lutgart ;Monsieur, Lieve ;Hinting, Aucky
- Publisher
- John Wiley and Sons
- Year
- 1989
- Weight
- 440 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
The measurement of the ATP content of fresh semen is as accurate as the estimation of sperm motility by conventional methods in discriminating between semen of fertile versus subfertile men. The ATP content of frozen thawed donor semen is correlated with the probability of conception per cycle of insemination. Exact quantification of cytotoxic sperm antibodies in serum is possible with the adenosine-triphosphate-release-cytotoxicity test, since measurement is free of the bias of microscopic examination. The procedure has been simplified by testing only one serum dilution and calculating the 'sperm toxicity index'.