Determination of acylglycerols in vegetable oil methyl esters by on-line normal phase LC-GC

✍ Scribed by Lechner, Marion ;Bauer-Plank, Christina ;Lorbeer, Eberhard

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 496 KB
• Volume: 20
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240201103

✦ Synopsis

An on-line LC-GC method for the analysis of mono-, di-, and triacylglycerols in vegetable oil methyl esters has been developed. The concentrations of these components have turned out to be key parameters for the quality of diesel fuel substitutes. Separation of all classes of acylglycerols from the fatty acid methyl ester matrix is achieved by LC after acetylation of the hydroxyl groups. The acylglycerol fraction is transferred on-line to GC, using the loop- type interface and concurrent eluent evaporation. Quantification of mono-, di-, and triacylglycerols is performed by combining external calibration with internal standardization. Both recovery of the procedure and reproducibility of the quantitative results are evaluated.