𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Determination of acyclovir in plasma by high performance liquid chromatography with UV detection. Method development and method validation

✍ Scribed by Šmidovnik, A. ;Wondra, Alenka Golc ;Prošek, Mirko

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
387 KB
Volume
20
Category
Article
ISSN
0935-6304

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A HJPLC method for the determination of acyclovir in plasma is described. The method is simple and sensitive enough for bioequiva‐lence studies, where a large number of plasma samples with low acyclovir concertration are involved. The procedure is based on the deproteinization of plasma with perchloric acid and separation of acyclovir on a Hypersil ODS Column at pH 5.6 with UV detection. The calibration standards are linear up to at least 4000 ng/mL and the limit of quantification is 10 ng/mL.

📜 SIMILAR VOLUMES

Determination of piceid in rat plasma an
Determination of piceid in rat plasma and tissues by high-performance liquid chromatographic method with UV detection
✍ Chunyan Lv; Lantong Zhang; Qiao Wang; Weina Liu; Chunying Wang; Xiujuan Jing; Ya 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 239 KB 👁 2 views

A rapid, sensitive and selective HPLC method was developed and validated for determination of piceid in rat plasma and tissues. The drug was isolated from plasma and tissues by a simple protein precipitation procedure. Chromatographic separation was performed on a C(18) column with acetonitrile-wate

Validation and pharmacokinetic applicati
Validation and pharmacokinetic application of a method for determination of doxazosin in human plasma by high-performance liquid chromatography
✍ Pattana Sripalakit; Penporn Nermhom; Aurasorn Saraphanchotiwitthaya 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 132 KB 👁 2 views

A simple high-performance liquid chromatographic method for the determination of doxazosin in human plasma was developed and validated. Prazosin was used as internal standard. After extraction twice with ethyl acetate, chromatographic separation of doxazosin in human plasma was carried out using a r