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Determination of acetylcholine and choline in the femtomole range by means of HPLC, a post-column enzyme reactor, and electrochemical detection

โœ Scribed by G. Damsma; D. Lammerts van Bueren; B. H. C. Westerink; A. S. Horn


Book ID
112827066
Publisher
Springer
Year
1987
Tongue
English
Weight
439 KB
Volume
24
Category
Article
ISSN
0009-5893

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HPLC detection of choline and acetylchol
โœ P. Van Zoonen; C. Gooijer; N.H. Velthorst; R.W. Frei; J.H. Wolf; J. Gerrits; F. ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 364 KB

A method using HPLC has been developed for the detection of choline (Ch) and acetylcholine (ACh) using an immobilized enzyme reactor which converts Ch and ACh into hydrogen peroxide and betaine. The formed H202 is quantified by means of a solid-state peroxyoxalate chemiluminescence detector based on