𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Determination of acetaldehyde in rat blood by the use of rat liver aldehyde dehydrogenase

✍ Scribed by Olof Tottmar; Hans Marchner; Hans Pettersson

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
600 KB
Volume
91
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A method has been developed for the determination of low concentrations of acetaldehyde in rat blood. The method involves extraction of blood in perchloric acid followed by a fluorimetric determination of acetaldehyde in neutralized extracts by the use of a low K(m) aldehyde dehydrogenase isolated from rat liver mitochondria. Acetaldehyde concentrations down to 2 to 3 microM could be detected in blood samples of 0.1 ml containing high concentrations of ethanol (10-40 mM). Due to its simplicity, sensitivity, and the use of a low-cost fluorimeter, this enzymatic method should be a valuable complement to gas chromatographic methods for acetaldehyde determination.

πŸ“œ SIMILAR VOLUMES

Inhibition of rat hepatic mitochondrial
Inhibition of rat hepatic mitochondrial aldehyde dehydrogenase–mediated acetaldehyde oxidation by trans-4-hydroxy-2-nonenal
✍ David Y. Mitchell; Dennis R. Petersen πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 820 KB

The hepatic oxidation of ethanol has been demonstrated to cause peroxidation of cellular membranes, resulting in the production of aldehydes that are substrates for hepatic aldehyde dehydrogenases. It was the purpose of this study to evaluate the cooxidation of the lipid peroxidation product, truns-

Lobular distribution of alcohol dehydrog
Lobular distribution of alcohol dehydrogenase in the rat liver
✍ Masayoshi Yamauchi; James J. Potter; Esteban Mezey πŸ“‚ Article πŸ“… 1988 πŸ› John Wiley and Sons 🌐 English βš– 729 KB

The hepatic lobular localization of alcohol dehydrogenase was determined in male, female and castrated male rats. Alcohol dehydrogenase immunoreactive protein and activity were increased in female and castrated rats as compared to normal male rats. By immunohistochemistry, alcohol dehydrogenase prot

Effect of pharmacologic interventions on
Effect of pharmacologic interventions on aldehyde dehydrogenase in the rat testicles
✍ F. S. Messiha πŸ“‚ Article πŸ“… 1981 πŸ› John Wiley and Sons 🌐 English βš– 303 KB

## Abstract The __in vitro__ and __in vivo__ effects of selected pharmacologic agents on cytosolic aldehyde dehydrogenase in the adult rat testicles were studied. Naltrexone, a narcotic antagonist, significantly inhibited epididymal but not testicular aldehyde dehydrogenase __in vitro.__ Greater __