Determination of 3J(1H3′31P) couplings in a DNA oligomer with enhanced sensitivity employing a constant-time TOCSY difference experiment
✍ Scribed by Lorenz M. Reith; Judith Schlagnitweit; Vilko Smrecki; Günther Knör; Norbert Müller; Wolfgang Schoefberger
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 172 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0749-1581
- DOI
- 10.1002/mrc.2729
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✦ Synopsis
Abstract
A constant‐time TOCSY difference experiment for the determination of ^3^J(^1^H3′^31^P) coupling constants in non‐isotope‐labelled DNA oligonucleotides is presented. The method is tested on a DNA octamer and compared with the established constant‐time NOESY difference method. Each ^3^J(^1^H3′^31^P) coupling constant is determined from amplitude changes caused by phosphorous decoupling, which are observable on multiple cross‐peaks, thus leading to a high accuracy of the value of the ^3^J(^1^H3′^31^P) coupling constant. The new experiment delivers up to three times the sensitivity compared with previously reported methods. Copyright © 2011 John Wiley & Sons, Ltd.