Determination and pharmacokinetics of manidipine in human plasma by HPLC/ESIMS

✍ Scribed by Jing Jing; Weichao Ren; Xijing Chen; Hui He; Wei Zhou; Xuping Zhu; Ya Sun; Guangji Wang

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 184 KB
• Volume: 21
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.827

✦ Synopsis

Abstract

A sensitive HPLC/ESIMS method was established for the determination of manidipine in human plasma and pharmacokinetics study. After basified plasma with ammonia, manidipine and the internal standard (IS) (felodipine) were extracted with n‐hexane and separated on a Hypersil ODS2 column with a mobile phase of methanol–5 mm ammonium acetate solution containing 0.1% acetic acid (85:15, v/v). MS determination was performed by electrospray ionization in the selected ion monitoring mode. Manidipine was monitored at m/z 611.4 and IS at m/z 384. The assay had a calibration range from 0.2 to 20 ng/mL and a lower limit of quantification of 0.1 ng/mL. The method has been successfully applied to the pharmacokinetic study in healthy volunteers. Copyright © 2007 John Wiley & Sons, Ltd.