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Determinants of the stimulation of fat cell adenylate cyclase by high concentrations of sodium and magnesium salts. Implications for the role of magnesium in regulation of enzyme activity

โœ Scribed by Michael S. Katz; John S. Partilla; Marco A. Pineyro; Robert I. Gregerman


Book ID
113124082
Publisher
Elsevier Science
Year
1980
Weight
531 KB
Volume
613
Category
Article
ISSN
0005-2744

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High and low affinity binding sites for
โœ David Moscatelli ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 834 KB

Scatchard analysis of binding of 125I-basic fibroblast growth factor (FGF) to baby hamster kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of 20 pM and 80,000 sites per cell and a low affinity site with KD of about 2 nM and 600,000 sites per cell. The bind