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Determinants of the selection of phosphatidylcholine molecular species for secretion into bile in the rat

✍ Scribed by Wayne W. LaMorte; Michael L. Booker; Susannah Kay

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
82 KB
Volume
28
Category
Article
ISSN
0270-9139

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✦ Synopsis

Abbreviations: PC, phosphatidylcholine; PC-TP, phosphatidylcholine transfer protein; HPLC, high-performance liquid chromatography; C1, the number of carbons in the sn-1 acyl chain of a PC; C2, the number of carbons in the sn-2 acyl chain of a PC; B1, the number of double bonds in the sn-1 acyl chain of a PC; B2, the number of double bonds in the sn-2 acyl chain of a PC.

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Employing the in situ perfused rat liver, we examined the origins and mechanisms of transport of proteins into bile. First, utilizing polyacrylamide gels, we noted that many biliary proteins co-migrated with dominant serum proteins. Upon liver perfusion with serum-free medium, most proteins disappea