✦ LIBER ✦
Detection reagent for adenine, guanine, uracil, cytosine and their alkylated bases, nucleotides and nucleosides on thin-layer plates
✍ Scribed by Haleem J. Issaq; Edward W. Barr
- Book ID
- 104144378
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 443 KB
- Volume
- 132
- Category
- Article
- ISSN
- 1873-3778
No coin nor oath required. For personal study only.
✦ Synopsis
The detection of alkylated bases at the nanogram level is reported. The reaction is based on two steps, first, chlorination of the bases and, secondly, sprsying the chlorinated product with o-tolidine solution to give a dark blue color. The-detection ofnucIeotides and nucleosides is also possible. The optimum conditions and the possible mechanism of the reaction are discussed.