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Detection ofVibrio choleraeO1 in the aquatic environment in Brazil employing direct immunofluorescence microscopy

✍ Scribed by M. T. Martins; P. S. Sanchez; M. I. Z. Sato; P. R. Brayton; R. R. Colwell


Book ID
104748486
Publisher
Springer
Year
1993
Tongue
English
Weight
311 KB
Volume
9
Category
Article
ISSN
1573-0972

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✦ Synopsis


Culturing

and immunofluorescence (FA) methods for detection of Vibrio cholerae 01 in samples collected from the aquatic environment at selected sites in Brazil were compared. Of the samples examined, 90% were positive for V. choferae 01 by FA but none was positive by culture, although strains of V. cholerae other than 01 strains were readily isolated. Evidence for V. cholerae 01 being autochthonous to the aquatic environment of Brazil is presented. Furthermore, FA methods are recommended for cholera surveillance programmes directed at the natural environment.