Detection of α-Glycosidic Bonds in the Ganglioside GM1 by Stereospecific Enzymatic Degradation

Cautionary note on the use of the B subu

Cautionary note on the use of the B subunit of cholera toxin as a ganglioside GM1 probe: Detection of cholera toxin A subunit in B subunit preparations by a sensitive adenylate cyclase assay

✍ Sarah Spiegel 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 591 KB

The use of the B subunit of cholera toxin, a protein that binds specifically to ganglioside GM 1, has provided a new paradigm for studying physiological functions of ganglioside GMl . The B subunit inhibited the growth of rat glioma C6 cells that had been pretreated with ganglioside GM1. In some pre