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Detection of α-amylase isoenzymes by means of two-dimensional electrophoresis followed by blue starch staining

✍ Scribed by Dr. Tsuyoki Kadofuku; Tsuneo Sato; Takashi Manabe; Tsuneo Okuyama

Publisher: John Wiley and Sons
Year: 1983
Tongue: English
Weight: 499 KB
Volume: 4
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.1150040611

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

α‐Amylase isoenzymes present in human body fluids were separated by two‐dimensional electrophoresis in the absence of denaturing agents and detected by blue starch staining. Human body fluid samples (5 sera, 2 salivas, 1 pancreatic juice and 1 urine) were subjected to the analysis. Two to six activity spots of α‐amylase were detected for each sample. The spots were classified into four main species by their isoelectric points (p__I__) 6.9, 6.5, 5.8 and 5.3. All the α‐amylase isoenzymes showed apparent molecular weights above 140 000.

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