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Detection of Y-bearing spermatozoa by DNA-DNA in situ hybridisation

✍ Scribed by Dr. John D. West; Katrine M. West; R. John Aitken

Book ID
112146941
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
627 KB
Volume
1
Category
Article
ISSN
1040-452X

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Detection of Y-bearing porcine spermatoz
Detection of Y-bearing porcine spermatozoa by in situ hybridization using digoxigenin-labeled, porcine male-specific DNA probe produced by polymerase chain reaction
✍ Tatsuo Kawarasaki; Tetsuya Kohsaka; Masaru Sone; Mitsutoshi Yoshida; Kimio Bamba πŸ“‚ Article πŸ“… 1995 πŸ› John Wiley and Sons 🌐 English βš– 492 KB

This study was carried out to determine whether Y-bearing porcine spermatozoa could be detected by in situ hybridization using a digoxigenin (Dig)-labelled DNA probe specific to the Y chromosome produced by polymerase chain reaction (PCR). A conventional PCR (with Dig-dUTP) was performed using a set