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Detection of transforming gene regions of human papillomavirus type 16 in cervical dysplasias by the polymerase chain reaction

✍ Scribed by J. Czeglédy; M. Evander; L. Veres; L. Gergely; G. Wadell

Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
636 KB
Volume
180
Category
Article
ISSN
0300-8584

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✦ Synopsis

In a study of 29 cases of histologically confirmed, characterized colposcopically and cytologically, cervical intraepithelial neoplasias 48.3% (14/ 29) of biopsies were positive for human papillomavirus (HPV) type 16 DNA by polymerase chain reaction. We used two oligonucleotide primer pairs (position 215-514 and 606-805) flanking a 300 and a 199 base pair fragment from the early 6 (E6) and early 7 (E7) genes. The results were concordant both with the E6 and with the E7 regions. Of the amplified products 85.7% (12/14) could be confirmed; these carried 16 specific sequences by Southern blot hybridization. HPV 16 DNA was present in 6.7% (2/30) of the colposcopically directed cytologically normal matched control samples using the same methods.

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## Abstract The polymerase chain reaction (PCR) was applied for the detection of human papilloma virus (HPV) infection, in samples obtained from the uterine cervices of 202 asymptomatic women with normal cytology in Northern Greece. About 41.8% of the women with microscopically and cytologically no