✍ Scribed by Keijiro Nakanishi; Isao Karube; Shingo Hiroshi; Arishune Uchida; Yuzaburo Ishida
No coin nor oath required. For personal study only.
Red tide is a phenomenon caused by small algae or phytoplankton where upon seawater appear> red. Both toxic and non-toxic red tides have occurred throughout recorded history but in recent years there has ken a global increase in the number of these events due to coastal pollution and to other unclear factors; for example, long-distance tra;.,m)rt of species across oceans. Some kinds of red tides cause mass deaths of cultured fish and thus cause serious ecc'n3r':li problems for countries where fish is one of the important protein supplies. In Japan, the Chattorr!Iu species is one of the dominant representatives among many kinds of flagellates recognized in red tides. In this paper, de'z.rmination of Chattonella marina was performed using a piezoelectric immunosensor comprising a gold-coated piezoelectric crystal coated with monoclonal antibody IviR-21. This antibody shows highly specific reactivity towards the cytoplasmic membrane of C. marina. The resonance frequency of the piezoelectric crystal is associated with a mass change at the surface and measurement of the frequency shift by reaction between the antibody m-21 imniobilized on the piezcelectric crystal and C. marina enables to detect C. marina. This immunosensor system is able to detect lo2 to IO4 cells ml-' of C. marina directly in seawater.
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