✦ LIBER ✦

Detection of the causal mutation for canine leukocyte adhesion deficiency (CLAD) using pyrosequencing

✍ Scribed by J M H Kijas; R K Juneja; S Gäfvert; L Andersson

Book ID: 104457342
Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 137 KB
Volume: 31
Category: Article
ISSN: 0268-9146
DOI: 10.1046/j.1365-2052.2000.00650.x

No coin nor oath required. For personal study only.

✦ Synopsis

A missense mutation in the ITGB2 gene causes canine leukocyte adhesion deficiency (CLAD) in Irish setters. We constructed a diagnostic test to identify heterozygous CLAD carriers based on a newly developed technology termed pyrosequencing. Although primarily designed for high‐speed generation of DNA sequence in a gel‐free system, the technology can be applied to rapid single‐nucleotide polymorphism analysis in a clinical setting. The testing of 339 dogs originating from a total of 10 countries was conducted and CLAD carriers were identified within every country where more than one sample was analysed. This indicates that the CLAD mutation is widespread and that there is a strong need for a robust diagnostic test.

📜 SIMILAR VOLUMES

Frequency of the canine leucocyte adhesi

Frequency of the canine leucocyte adhesion deficiency (CLAD) mutation among Irish red setters in Germany

✍ I. Pfeiffer; B. Brenig 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 57 KB

Lentiviral vectors incorporating a human

Lentiviral vectors incorporating a human elongation factor 1α promoter for the treatment of canine leukocyte adhesion deficiency

✍ Nelson, E J R; Tuschong, L M; Hunter, M J; Bauer, T R; Burkholder, T H; Hickstei 📂 Article 📅 2010 🏛 Nature Publishing Group 🌐 English ⚖ 459 KB

Canine Leukocyte Adhesion Deficiency: Pr

Canine Leukocyte Adhesion Deficiency: Presence of the Cys36Ser β-2 Integrin Mutation in an Affected US Irish Setter Cross-Breed Dog and in US Irish Red and White Setters

✍ Polly Foureman; Mary Whiteley; Urs Giger 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 418 KB