Detection of the biotin–streptavidin interaction by exploiting surface stress changes on ultrathin Si membranes
✍ Scribed by Vasiliki Tsouti; Christos Boutopoulos; Peristera Andreakou; Marina Ioannou; Ioanna Zergioti; Dimitris Goustouridis; Dimitris Kafetzopoulos; Dimitris Tsoukalas; Pascal Normand; Stavros Chatzandroulis
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 548 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0167-9317
No coin nor oath required. For personal study only.
✦ Synopsis
The detection of the biotin-streptavidin interaction is presented based on surface stress changes of functionalized Si membranes. During the biomolecular interaction, the surface stress of the functionalized membranes alters resulting in their deflection and finally in capacitance variations. The biological interaction on the sensors surface was verified after each experiment using fluorescent microscopy. Successful detection of 21 nM of streptavidin using 3.55 mM biotin probes is presented.