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Detection of the basic fibroblast growth factor low affinity binding site in cardiac sarcolemmal vesicles

โœ Scribed by Chris R. Ross; Calvin C. Hale


Book ID
118344172
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
538 KB
Volume
46
Category
Article
ISSN
0024-3205

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High and low affinity binding sites for
โœ David Moscatelli ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 834 KB

Scatchard analysis of binding of 125I-basic fibroblast growth factor (FGF) to baby hamster kidney (BHK) cells revealed the presence of two binding sites: a high affinity site with KD of 20 pM and 80,000 sites per cell and a low affinity site with KD of about 2 nM and 600,000 sites per cell. The bind