Determination of particle number and bri
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Rooshin B. Dalal; Michelle A. Digman; Alan F. Horwitz; Valeria Vetri; Enrico Gra
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Article
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2007
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John Wiley and Sons
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English
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## Abstract We describe a method to obtain the brightness and number of molecules at each pixel of an image stack obtained with a laser scanning microscope. The method is based on intensity fluctuations due to the diffusion of molecules in a pixel. For a detector operating in the analog mode, the v