Detection of sub-nanogram quantities of Mojave toxin via enzyme immunoassay with light addressable potentiometric detector
✍ Scribed by James T. Colston; Pramod Kumar; Epple D. Rael; Andrew T.C. Tsin; James J. Valdes; James P. Chambers
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 477 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0956-5663
No coin nor oath required. For personal study only.
✦ Synopsis
Sensitive detection of Mojave toxin (MoTX), a potent neurotoxin isolated from the venom of Crotalus scutulatus scutulatus, was developed using an enzyme immunoassay (EIA) and light addressable potentiometric detection. This EIA utilizes both biotin- and fluorescein-labeled anti-MoTX antibodies to immobilize and detect sub-nanogram to nanogram quantities of toxin. Labeled mono- and polyclonal anti-MoTX antibodies were used alone and/or in combination to determine maximum assay sensitivity. Assays performed using a combination of biotinylated poly- and fluoresceinated monoclonal antibodies produced an assay with a lower detection limit near 2.5 ng. Assays performed using labeled polyclonals alone, or in combination with biotinylated mono- and fluoresceinated polyclonal antibodies, indicated increased sensitivity with a detection limit near 300 pg. In conclusion, we describe an enzyme immunoassay using different labeled antibody schemes which detects sub-nanogram quantities of MoTX via light addressable potentiometric detection.