Detection of Stromelysin and Collagenase in Synovial Fluid From Patients with Rheumatoid Arthritis and Posttraumatic Knee Injury

✍ Scribed by Lori A. Walakovits; Vernon L. Moore; Nina Bhardwaj; Gregory S. Gallick; Michael W. Lark

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 742 KB
• Volume: 35
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780350106

✦ Synopsis

To quantify stromelysin and collagenase in synovial fluid (SF) from patients with rheumatoid arthritis (RA) or traumatic knee injury.

Methods. Stromelysin and collagenase were measured in the SF of 33 patients with RA or pttraumatic knee injury, using specific double-antibody sandwich enzyme-linked immunosorbent assays. Stromelysin was fractionated from representative SF, and the molecular form was identilied by immunoblot analysis.

Results. The stromelysin concentration was -20fold higher than the collagenase concentration in the fluids from patients with RA and -8-fold higher in the fluids from patients with traumatic iaury. For both metalloproteinases, there was a higher enzyme concentration in RA SF than in the SF from patients with trauma (stromelysin 40.1 f 26 pg/ml [mean f SDI in RA SF, 8.5 +: 15 d m l in trauma SF; collagenase 2.2 f 3.3 pg/ml in RA SF, 1.1 f 2.3 pg/ml in trauma SF).

The majority of the stromelysin within the SF bound to reactive red-agarose and was identified as prostrome-From the