Detection of Staphylococcus aureus with a fluorescence in situ hybridization that does not require lysostaphin

✍ Scribed by Thomas S. Lawson; Russell E. Connally; Jonathan R. Iredell; Subramanyam Vemulpad; James A. Piper

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 118 KB
• Volume: 25
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.20448

✦ Synopsis

Abstract

To detect with whole‐cell fluorescence in situ hybridization (FISH), Staphylococcus aureus is typically permeabilized with lyozyme and lysostaphin. We tested whether it was feasible to detect S. aureus and differentiate it from Staphylococcus epidermidis with lysozyme‐only permeabilization. We compared lysozyme permeabilizationto S. aureus permeabilized with lysozyme in combination with lysostaphin. It was determined that S. aureus treated with agarose, methanol, and lysozyme could be detected with FISH. The 1 hr protocol is a useful alternative to conventional FISH. J. Clin. Lab. Anal. 25:142–147, 2011. © 2011 Wiley‐Liss, Inc.