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Detection of specific binding of [1,6-3H]2,3,7,8-tetrachlorodibenzo-p-dioxin ([3H]TCDD) in human leukocytes using electrofocusing in polyacrylamide gel

✍ Scribed by Gillner, Mikael; Haldosén, Lars-Arne; Gustafsson, Sven A.; Gustafsson, Jan-Åke


Book ID
123156435
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
676 KB
Volume
47
Category
Article
ISSN
0378-4274

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Filtration assay for quantitation of 2,3
✍ Karen M. Dold; William F. Greenlee 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 756 KB

A rapid and sensitive filtration assay for quantitating the specific binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to whole cells in culture is described. Cell monolayers are incubated with [3H]TCDD in the presence or absence of excess unlabeled ligand, detached from the culture dish with tr