Detection of somatic changes in human renal cell carcinomas with oligonucleotide probes specific for simple repeat motifs
✍ Scribed by Susanne Bock; Jörg T. Epplen; Heidi Noll-Puchta; Michael Rotter; Heinz Höfler; Thomas Block; Rudolph Hartung; Gerhard Jakse; Wolfgang Wilmanns; Petro E. Petrides
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 418 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1045-2257
No coin nor oath required. For personal study only.
✦ Synopsis
The purpose of our study was to1 detect somatic changes in renal cell carcinoma by multilocus fingerprinting. DNA fingerprints were generated from the DNA of normal and malignant renal tissue samples of 29 patients with nonhereditary kidney carcinoma by using oligonucleotide probes specific for simple repeat motifs such as (GTG),, (CA),, (GACA),, or (TTAGGG),. Each probe rendered a typical fingerprint pattern, because it is specific with respect t o the target regions recognized in the genome. The restriction enzymes used were Hinfl and Haelll. Changed banding patterns were detected by using (GTG), in 20% of the tumors, in 20% for (CA), after Hinfl digestion, and in 10% after Haelll digestion. Even more informative probes were (GACA),, showing 70% changes after Haelll digestion, and (TTAGGG),, with 80% changes after digestion with either enzyme.
Since the simple repeat motifs riecognized by (GACA), are localized on the short arms of the acrocentric chromosomes (I 3, 14, IS, 2 I, and 22). it is possible t.hat sequences important for renal carcinogenesis are present in these regions. The observation of changes in regions t o which (TTAGGG), hybridizes points t o an involvement of DNA elements in telomeric sequence related regions in human kidney tumor formation. Genes Chrom Cancer 611 13-1 17 (1993).