Detection of small GTP-binding proteins in the outer envelope membrane of pea chloroplasts
β Scribed by Yukiko Sasaki; Kazumasa Sekiguchi; Yukio Nagano; Ryuichi Matsuno
- Book ID
- 115925429
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 340 KB
- Volume
- 293
- Category
- Article
- ISSN
- 0014-5793
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Three GTPβbinding proteins with apparent molecular masses of 27, 28 and 30 kDa have been detected in isolated nuclei of etiolated pea plumules. After LDSβPAGE and transfer to nitrocellulose these proteins bind [^32^p]GTP in the presence of excess ATP, suggesting that they are monomeric G proteins. W
## Abstract Mitochondria and crude nuclei containing fractions from human placenta have been shown to contain proteins which bind [Ξ±^32^P]βGTP. Prior to this study the number of GTPβbinding proteins in placental nuclei and their nucleotide specificity was not known. Also unknown was the identity of