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Detection of sister chromatid exchanges by 4′-6-diamidino-2-phenylindole fluorescence

✍ Scribed by M. S. Lin; O. S. Alfi

Publisher: Springer
Year: 1976
Tongue: English
Weight: 696 KB
Volume: 57
Category: Article
ISSN: 0009-5915
DOI: 10.1007/bf00295208

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✦ Synopsis

This paper describes a 4'-6-diamidino-2-phenylindole (DAPI) fluorescent technique for differentiation of sister chromatids and for the study of sister chromatid exchanges (SCE) in mouse chromosomes. The advantages of the DAPI fluorescent technique are also described. Differences in the occurrence of SCE between the centromeric heterochromatin (C-banded) and the chromosomal arm chromatin were studied in mouse cells (RAG) with or without mitomycin C treatment. Single strand exchanges between the DNA double helices in the sister chromatids were not detected. SCE and chromosome breakage appeared to occur more frequently in the centromeric region than in the chromosomal arm. This might play an important role in chromosome evolution in mice.

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