Detection of Reovirus Type 3 in the Porta Hepatis of an Infant with Extrahepatic Biliary Atresia: Ultrastructural and Immunocytochemical Study
✍ Scribed by Rachel Morecki; Joy H. Glaser; Anne B. Johnson; Yvonne Kress
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 713 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0270-9139
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✦ Synopsis
This report describes immunocytochemical and ultrastructural methods which led to the identification of Reovirus type 3 (Reo-3) in the porta hepatis of a patient with extrahepatic biliary atresia. The study indicates that Reo-3 antigenic sites are demonstrable by the avidin-biotinylated complex peroxidase method following formalin fixation and paraffin embedding, but are destroyed by freezing and thawing prior to fixation. Deparaffinization of the block and subsequent rembedding in epon-araldite did not alter immunoperoxidase staining. This procedure offered the advantage of higher light microscopic resolution of semithin (1 rm) sections and assisted in the selection of specific areas for ultrastructural studies.
Localization of Reo-3 in extrahepatic biliary atresia was confined to a biliary remnant in which there were acutely inflammed, partially necrotic microscopic ducts. Electron microscopic examination of the immunoreactive sites revealed virus-like particles similar in appearance to Reo-3 particles in infected tissue culture cells. The observations presented here support previously reported serologic data which have shown an association between Reo-3 infection and extrahepatic biliary atresia.
Reovirus type 3 (Reo-3) has been implicated as an etiologic agent in extrahepatic biliary atresia (EHBA), and more recently in neonatal hepatitis. This association is based on serologic studies which indicate that in the first year of life, Reo-3 antibodies are elevated in over 60% of patients with EHBA, and in over 50% with neonatal hepatitis. In contrast, they are rarely found in age-matched healthy controls or controls with cholestasis due to other causes (1, 2). Further indication that a perinatal infection with Reo-3 may cause EHBA is provided by observations in mice. Experimental infection of weanling mice with Reo-3 results in acute hepatobiliary inflammation which evolves into a chronic icteric disease with obliterative inflammation of extraheptic bile ducts resembling human EHBA (3,4). A common observation in the murine and human disease is the failure to identify