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Detection of recombination events in human cells using cloned cytomegalovirus DNA fragments as probes

✍ Scribed by Hamelin, Claude; Yelle, Jocelyn; Dion, Michel


Book ID
123271995
Publisher
Elsevier Science
Year
1988
Weight
381 KB
Volume
5
Category
Article
ISSN
0735-0651

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## Abstract A biotin‐labeled DNA probe specific for the im‐ mediate early gene of the cytomegalovirus (CMV) strain AD 169 was generated with the polymerase chain reaction. Nucleic acid hybridisation was carried out with CMV‐infected T‐lymphoblastoid cells (MOLT4) after 4 hr to 6 days of culture. Bi