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Detection of pyridinium cross-links in human bile

✍ Scribed by M. Romanello; K. Noris Suarez; P. Bettica; L. Moro

Publisher: Springer
Year: 1995
Tongue: English
Weight: 361 KB
Volume: 57
Category: Article
ISSN: 1432-0827
DOI: 10.1007/bf00301942

No coin nor oath required. For personal study only.

✦ Synopsis

This study evaluated whether pyridinium cross-links, which are positively charged, besides renal clearance are also cleared by the liver into bile. In 13 human bile samples tested, we were able to detect both pyridinoline (PYD) and deoxypyridinoline (DPD) in small amounts which were estimated to be about 1-2% of the amount usually found in urine. To further evaluate the amount of pyridinium cross-links excreted through bile, we studied the stability of these compounds at the alkaline pH of bile. No effect on their stability was detected over a 6-hour incubation. The origin of these molecules in bile and the significance of this finding in the use of PYD and DPD as bone resorption markers are discussed.

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