Detection of protein–ligand interactions by NMR using reductive methylation of lysine residues
✍ Scribed by Sherwin J. Abraham; Susanne Hoheisel; Vadim Gaponenko
- Book ID
- 106401580
- Publisher
- Springer Netherlands
- Year
- 2008
- Tongue
- English
- Weight
- 231 KB
- Volume
- 42
- Category
- Article
- ISSN
- 0925-2738
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## Abstract Saturation transfer difference (STD)‐NMR has been widely used to screen ligand compound libraries for their binding activities to proteins and to determine the binding epitopes of the ligands. We report herein, a Clean STD‐NMR method developed to overcome false positives (artifacts) obs
The interaction of methyl orange with poly(L-lysine) was studied kinetically by the stopped-flow technique with CD detection, as well as by static CD titration experiments. In the static experiments, the differences observed in the polymer-to-dye ratio dependences of the CD spectra and absorption sp