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Detection of preferential NRAS mutations in human male germ cell tumors by the polymerase chain reaction

✍ Scribed by Dr. Sabyasachi Ganguly; Vundavalli V. V. S. Murty; Felipe Samaniego; Victor E. Reuter; George J. Bosl; R. S. K. Chaganti

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
383 KB
Volume
1
Category
Article
ISSN
1045-2257

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✦ Synopsis

We have studied 3 I male germ cell tumors (GCTs) for probable mutations in codons 12, 13, and 6 I of HRAS, KRAS, and NRAS oncogenes using the polymerase chain reaction. Twenty of the thirty-one tumors exhibited NRAS gene mutations, 14 in codon 6 I, and six in codon 12, whereas no mutations were detected in HRAS and KRAS genes. The NRAS mutations were equally prevalent in seminomatous and nonseminomatous GCTs. Thus I 3 of 22 seminomas, six of seven embryonal carcinomas, and one of two mixed tumors exhibited mutations. Two non-seminomatous tumors (an embryonal carcinoma and a yolk sackeratoma) had mutations in both codons I 2 and 6 I. The high frequency of NRAS mutations observed in the present study suggests that NRAS gene products may play an important role in growth regulatory functions of premalignant and malignant germ cells.

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