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Detection of polygalacturonase and pectin lyase isoenzymes in polyacrylamide gels

✍ Scribed by N. Lisker; Nira Retig


Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
347 KB
Volume
96
Category
Article
ISSN
1873-3778

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## Abstract A simple and versatile method was developed for detecting endoproteinases by nondenaturing polyacrylamide gel electrophoresis. A vertical strip of the resolving gel is subjected horizontally to a second electrophoresis in the presence of a substrate protein and sodium dodecyl sulfate (S