Detection of Poly(ADP-Ribose) Polymerase and Its Apoptosis-Specific Fragment by a Nonisotopic Activity–Western Blot Technique
✍ Scribed by Girish M. Shah; Scott H. Kaufmann; Guy G. Poirier
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 95 KB
- Volume
- 232
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
underestimated values for the AT duplexes (GC £ 50%, nucleotide oligos) Eq. [3] also gave similar satisfactory results (see Fig. ). AT § 50%). Deviations between predicted and experimental T m values are more scattered when Eqs. and
The data presented here indicate that the thermal stability for shorter oligonucleotides (£18) seems to be [2] are applied compared with Eq. [3] (Fig. ). Results also show that all three equations overestimated the independent of the GC content but is dependent on the length of the oligo. predicted T m values for oligonucleotide lengths higher than 20 nt (Fig. , A2 to C2).
Acknowledgments.