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Detection of point mutations using pyrene-labeled DNA probes

✍ Scribed by N.N. Dioubankova; A.D. Malakhov; D.A. Stetsenko; V.A. Korshun

Book ID
111607634
Publisher
Springer
Year
2004
Tongue
English
Weight
519 KB
Volume
53
Category
Article
ISSN
1573-9171

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## Abstract **Powerful pyrene probes**: Two kinds of pyrene‐labeled oligonucleotides (HNA‐ and RNA‐skeleton probes) were explored. The enhanced fluorescence intensity in the monomer region and the disappearance of aggregate/excimer emission in duplexes has been successfully used to detect the hybri