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Detection of point mutations of the Axin1 gene in colorectal cancers

✍ Scribed by Li-Hua Jin; Qiu-Jie Shao; Wen Luo; Zhi-Yun Ye; Qing Li; Sheng-Cai Lin

Publisher: John Wiley and Sons
Year: 2003
Tongue: French
Weight: 433 KB
Volume: 107
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.11435

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Axin is a recently identified tumor suppressor that plays an important role in liver and colon cancers. To gain further insights into the structure and function of Axin in controlling cell growth, we analyzed 54 colorectal cancer tissues for mutations in AXIN1 gene. We employed PCR amplification with 23 sets of primers against introns that encompassed the whole coding region of AXIN1 followed by single‐strand conformation polymorphism (SSCP) analysis. After subcloning and sequencing analysis of the reamplified DNA from the aberrant bands, we found, in addition to 3 silent mutations, 6 misssense point mutations in different functionally important regions. The missense mutation rate is hence 11%, suggesting that Axin deficiency may contribute to the onset of colorectal tumorigenesis. © 2003 Wiley‐Liss, Inc.

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